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Hydrocarbon degradation potential of indigenous fungal isolates from Indian oil refinery, Haldia, (W. B.) India
Shreyasri Dutta* and Padma Singh
Microbiology Department, Kanya Gurukul Campus, Gurukul Kangri University, Haridwar-249407
Application of fungus for effective removal of hydrocarbon contamination from soil has been considered as an effective way. In present study contaminated soil samples from three sites of Indian oil refinery, Haldia, W.B were studied for assessment of their refine oil degrading potential. The average refines oil utilizing fungi varied from 4.13X102+0.88CFU/g to 3X102+0.61CFU/g. Nine fungal isolates recovered from oil-contaminated soils were screened for refine oil biodegradation activity in a shake-flask culture by using 2, 6-DCPIP as a indicator. Among the nine fungal isolates, only five showed potential for biodegradation. The identified fungal genera included Aspergillus flavus, Aspergillus terrus, Aspergillus niger, Penicillium sp. and Gleocladium sp. Biodegradation ability of all isolates was confirmed by vapour phase transfer and CO2 evolution method. The results showed that indigenous fungal isolates displayed highest capability for biodegradation of refine petroleum oil. Hence, these fungal species can be used for bioremediation of petroleum contaminated sites.
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