Current Issue Volume 5, Number 1 (April - 2015) ...
Latest Updates
Current Issue of Bioscience Discovery Published
Volume 6, No. 1, Jan. 2015
Quick Links:
Bioscience Discovery
An International Journal of Life Sciences
Available on

Culture characteristics and aflatoxin producing strains of Aspergillus flavus from maize grains

Anil U. Kulkarni1* and Ashok M. Chavan

1Lalbahadur Shastri Senior College, Partur Dist. Jalna-431501 (M.S.) India
Department of Botany, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad -431004 (M.S.) India

The known mycotoxin the most important which have been studied mostly because of possible hazards to human health are the afltoxins.Aflatoxins produced very commonly by two species of Aspergillus viz. Aspergillus flavus and Aspergillus parasitic. In this study growth pattern and culture characteristics of Aspergillus flavus isolates were studied. Twelve strains of A. flavus were isolated from three varieties of maize seed. To study the growth pattern of different isolates of A.flavus, CZA and GNA media were used. The different strains of A.flavus showed great variation in diameter of colony and growth pattern. There was also variation in the colour of colony from both sides.  Aspergillus flavus is the most important aflatoxin producing mould. This species in maize produces carcinogenic, mutagenic and teratogenic secondary metabolites. Estimation of aflatoxin is generally monitored by ELISA & HPTLC.. Twelve isolates of Aspergillus flavus isolated from three maize varieties were tested by cultural method for aflatoxin detection, which is inexpensive and rapid. Two isolates viz. AF1 and AF12 were found highly toxic which showed dark pink colour development ,while AF2 were found moderately toxigenic ,because these isolates turned into moderate pink color, while eight isolates viz. AF3, AF4, AF5, AF7, AF8, AF9, AF10, AF11 exhibited light pink colour after treatment with ammonium vapor ,were low toxigenic. Among all the isolates tested only one viz. AF6 isolate were found to be nontoxigenic. The results of ammonia vapors test were up to 90% with the result of toxin detection by high pressure thin layer chromatography (HPTLC).

FULL TEXT (Click here)

Bennett, JW & Papa KE, 1988. The aflatoxigenic Aspergillus spp. Advance in plant pathology, 6. 263-280.
Diener, UL & ND Davis, 1966. Aflatoxin production by isolates of Aspergillus flavus. Phytopathology, 56: 1390-1393.
Dorner JW, 2002. Simultaneous quantification of Aspergillus flavus, A. parasiticus and aflatoxin in peanuts. Journal of AOAC international, 85: 911-916.
Jaimez J Ordaz, BI Vazquez, CM Franco & A Cepeda, 2001. New Additive for culture media for rapid identification of Aflatoxin producing Appl. Environ. Microbioz, 67: 4858-4862.
Kumar S, Shekhar M, Khan AA & Sharma P, 2007. A rapid technique for detection of toxigenic and non toxigenic strains of Aspergillus flavus from maize grains. Indian Phytopath. 60 (1): 31-34.                 
Mahendra KR, Bonde S, Ingale AP &  Gade AK, 2012. Mycotoxin: Rapid  detection , differentiation and  safety. J. Pharm  Edu. Res., 3: 22-34.
Morgos CM & Thompson VS, 1999. Fiber optic immunosensor for mycotoxins. Natural toxins 7: 371-376.
Mogle UP, 2013. Efficacy of leaf extracts against the Post-harvest fungal pathogens of Cowpea. Bioscience Discovery, 4(1):39-42.
Mogle UP, 2014. Influence of Weed Green Manure on production and Nutrient content of Maize cultivar. Journal of AgriSearch 1(4): 210-215.
Prakash HS & Udaya S, 2009. Molecular bar coding of seed borne pathogens. 5th International conference on plant pathology in the globalize era, New Delhi, pp33.
Saito V & Machida S, 1999. A rapid identification method for aflatoxin producing strains of Aspergills flavus and A. parasiticus by ammonia vapour. Mycoscience, 40: 205-211.
Zain ME, 2011. Impact of mycotoxins on human and animals. Journal of Squdi Chemical Society, 15: 129-144.

How to Cite this Article:
Anil U. Kulkarni and Ashok M. Chavan, 2015. Culture characteristics and aflatoxin producing strains of Aspergillus flavus from maize grains. Science Research Reporter, 5(1):24-29.

Editor: Dr. Umesh P. Mogle, Printed, Published and Owned by Dr. Umesh P. Mogle, Published from ‘Kaushalyaaie’, P. No. 43, Priyanka Residancy, Mantha Chaufulli Jalna 431203 (M.S.) India.

Quick Link to Bioscience Discovery

Bioscience Discovery home
Instructions to Author
Submission of Research Paper Current Issue

Photo Gallery

Follow Us (Social Sites)