In Silico Analysis of HMG CO-A Reductase of Candida albicans SC5314

Santosh Kodgire, Vikram Pawar, Nilesh Wagh, Laxmikant Kamble and Gajanan Zore*

School of Life Sciences, SRTM University, Nanded-431606
gbzore@rediffmail.com

Abstract
In the present study In silico comparative analysis of HMG CoA reductases (HMGCR) from Candida albicans and Homo sapiens were done. True orthologs of HMGCR from C. albicans, H. sapiens with percent protein similarity of 52 % and 34 % respectively to that of functionally characterized HMGCR from Schizaosaccharomyces pombe were found out. Conserved domains and motifs on these proteins was identified namely HPIH, MMPL superfamily and HMGCR superfamily. Out of these domains, the domain HPIH (4 to 154 amino acid) found in C. albicans was absent in H. sapiens. 3D protein analysis showed considerable differences in the structures of C. albicans and human HMG CoA reductases although amino acids TYR, GLU and ASN at catalytic domains are conserved.  The position of these amino acids in ligand binding site of HMGCR of C. albicans is different than that found in human HMGCR. Phylogenetic analysis with respect to HMGCR reveals that C. albicans lies apart from H. sapiens. Thus this was an attempt to identify and understand structure, putative function and evolution of true ortholog of HMGCR from C. albicans, a pet target for cholesterol lowering drugs in humans by a detailed bioinformatics analysis.

FULL TEXT (Click here)

REFERENCES
Armon A, 2001. ConSurf: an algorithmic tool for the identification of functional regions in proteins by surface mapping of phylogenetic information. J Mol Biol. 307: 447-463.
Basson ME, 2009. Saccharomyces cerevisiae contains two functional genes encoding 3-hydroxy-3-methylglutaryl-coenzyme A reductase. Proc. Natl. Acad. Sci. USA. 83: 5563-5567.
Berman J, 2006. Morphogenesis and cell cycle progression in Candida albicans. Curr. Opin. in Microbiol. 9 (6): 595-601.
Calderone RA and Fonzi WA, 2001. Virulence factors of Candida albicans. Trends Microbiol. 9: 327-35.
Cutler JE, 1991. Putative virulence factors of Candida albicans. Annu Rev Microbiol. 45: 187-218.
Dhaliwal AK, 2014. Comparative analysis of ABCB1 reveals novel structural and functional conservation between monocots and dicots. Front Plant Sci. 5: 1-10.
Eiglmeier K, 2005. Comparative analysis of BAC and whole genome shotgun sequences from an Anopheles gambiae region related to Plasmodium encapsulation. Insect Biochem Mol Biol. 35 (8): 799­814.
Goli S, 2013. In Silico characterization and comparative analysis of PTEN in different species
involved in nsclc. Trends Life Sci. 2 (1): 26-31.
Kelley LA, 2010. Protein structure prediction on the Web: a case study using the Phyre server. Nature Prot. 4 (3): 363-371.
Kevin MD, 2004. Plant pigments and their manipulation. Annu. Plant Rev. 14: 1-342.
Koning AJ, 1996. Different subcellular localization of Saccharomyces cerevisiae HMG-CoA reductase isozymes at elevated levels corresponds to distinct endoplasmic reticulum membrane proliferations. Mol Biol Cell. 7 (5):769-89.

Editor: Dr. Umesh P. Mogle, Printed, Published and Owned by Dr. Umesh P. Mogle, Published from ‘Kaushalyaaie’, P. No. 43, Priyanka Residancy, Mantha Chaufulli Jalna 431203 (M.S.) India.