Partial characterization of protease from Bacillus flexus

Tambekar DH*, Sandhya Tambekar1 and  Jadhao MR

*PG Dept. of Microbiology, SGB Amravati University, Amravati, 444602 (India)
1Dept. of Microbiology, Dhote Bandhu Science College, Gondia, 441614
*diliptambekar@rediffmail.com

Abstract
Halo-alkaliphiles grow high pH and high salt concentration can be a source of novel enzymes. The enzymes produced by these bacteria have great importance in industry due to its high thermo and pH stability. The alkaline protease enzymes have great importance in industries due to its thermo and pH stability. The alkaline protease producing bacteria are generally found in sea water or alkaline lakes such as Lonar Lake. Present study deals with the isolation, characterization, production dynamics and optimization of protease from bacteria. A total  of  6 bacterial cultures were isolated from alkaline Lonar  lake,  one strain  DHT13 showed prominent  proteolytic  activity which was  studied further for its phenotypic and biochemical characters. The bacterium DHT13 was screened for production and partial characterizations of protease, and 16SrRNA sequencings identified as Bacillus flexus. Protease from Bacillus flexus is active at high temperature of 800C and pH 10 and finds potential applications in food, pharmaceutical, leather and detergent industries.

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How to Cite this Article:
Tambekar DH, Sandhya Tambekar and  Jadhao MR, 2015. Partial characterization of protease from Bacillus flexus. Science Research Reporter, 5(1):92-96.

Editor: Dr. Umesh P. Mogle, Printed, Published and Owned by Dr. Umesh P. Mogle, Published from ‘Kaushalyaaie’, P. No. 43, Priyanka Residancy, Mantha Chaufulli Jalna 431203 (M.S.) India.