GC - MS determination of bioactive components of Gracilaria dura (C.Agardh) J. Agardh

Biji Cyriac1 and K. Eswaran 2,3*

1 Department of Zoology, Fatima College, Madurai - 6250 017, Tamil Nadu, India
2Marine Algal Research Station, CSIR-Central Salt & Marine Chemicals Research Institute,
Mandapam Camp – 623 519, Tamil Nadu, India
3Academy of Scientific and Innovative Research (ASIR), CSIR, New Delhi –110 001, India
*Corresponding author: Tel: +91-4573-241422, 241921; fax: +91-4573-241422,
*eswaran@csmcri.org

Article Info		Abstract
Received: 07-08-2015, Revised: 25-09-2015, Accepted: 27-09-2015		Marine algae are known to produce a wide variety of bioactive secondary metabolites which gives prospective scope of novel drug designing in pharmaceutical industry. The Gracilaria dura belongs to the family Gracilariaceae is an important medicinal and food taxon. Thirteen compounds were identified in the methanol extracts of G. duraand the major components are n-Hexadecanoic acid, Oleic Acid, Pytol, Squalene, 1,2 Benzenedicarboxylic acid, mono (2-ethylhexyl) ester, 9-Octodecenoic acid, palmitoleic acid etc and found to have antimicrobial, antioxidant, anti-inflammatory, antitumor, anti-androgenic and cancer preventive properties.  The findings of the present study confirm the presence of important phytocompounds in G.dura and helpful for further detailed study to develop the drugs from marine algae to alleviate various diseases.
Keywords: Gracilaria dura, GC-MS analysis, phytochemicals, Hexadecanoic acid, Oleic Acid, Squalene

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REFERENCES
LITERATURE CITED                                                               
Barbosa JP,  Fleury BG,  da-Gama BAP,  Teixeira VL and Pereira RC, 2007.Natural products as antifoulants in the Brazilian brown alga Dictyotapfaffii (Phaeophyta, Dictyotales). Biochem. Syst. Ecol.35 (8): 549-553.
Baghel RS,  Kumari P,  Bijo AJ,  Gupta V,  Reddy CRK  and Jha B, 2011. Genetic analysis and marker assisted identification of the life phases of red alga Gracilaria corticata (J. Agardh). Mol. Biol. Rep. 38: 4211-4218.
Bazes A,  Silkina A,  Douzenel P,  Fay F,  Kervarec N,  Morin D,  Berge J  and Bourgougnon N, 2009. Investigation of the antifouling constituents from the brown alga Sargassum miticum (Yendo) fenshott. J. Appl. Phycol. 21(4): 395-403.
Bhogaonkar PY, Chavhan VN, and Kanerkar UR, 2014.Nutritional potential of Ficusracemosa L. Fruits. Bioscience Discovery, 5(2): 150-153.
Blouin NA,  Brodiem JA,  Grossmanm AC,  Xu P and Brawley SH, 2011.Porphyra: a marine crop shaped by stress. Trends plant Sci. 16: 29-37.
Daffodil ED, Uthayakumari, FK and Mohan VR, 2012. GC-MS determination of bioactive compounds of Curculigo orchioides gaertn. Science Research Reporter, 2(3): 198-201.
Gressler V, Yokoya NS,  Fujii MT,   Colepicolo P,  Mancini J,  Torres RP and Pinto E, 2010. Lipid, fatty acid, protein, amino acids and ash content in four Brazilian red algae species. Food Chem. 120: 585-590.
Harada H, Yamashita U,  Kurihara H,  Fukushi E, Kawabata J and  Kamei Y, 2002. Antitumor activity of palmtic acid found as a selective cytotoxic substance in a marine red alga. Anticancer Res. 22: 2587-2590.
Hema R,  Kumaravel S,  Gomathi S and Sivasubramaninam C, 2010. Gas Chromatography mass Spectroscopic analysis of Lawsoniainermis leaves. New York Sci. J., pp: 141-143.
Holdt SL  and Kraan S  2011. Bioactive compounds in seaweed: functional food applications and legislation. J. ApplPhycol 23: 543-597.
Hung CC, Gong GC, Chung WC,  Kuo WT  and Lin FC, 2009. Enhancement of particulate organic carbon export flux induced by atmospheric forcing in the subtropical oligo - trophic northwest Pacific ocean. Mar. Chem. 113: 19-24.
Khotimchenko SV, 2005. Lipids from the marine alga Gracilariaverrrucosa. Chem. Nat. Comp. 41: 285-288.
Kulkarani  S, Kulkarani DK,  Deo AD,  Pande AB, and Bhagat R l, 2014. Use of ethano veterinary medicines (EVM) from Vidarbha region (MS) India. Bioscience Discovery. 5(2): 180-186.
Kumar M,  Kumari P,  Trivedi  N,  Shukla MK,  Gupta V,  Reddy CRK  and Jha B, 2011.  Minerals, PUFSs and antioxidant properties of some tropical seaweed from Saurashtra coast of India. J Appl. Phycol. 23: 797-810.
Manilal A,  Sujith S,  Kiran GS,  Selvin J  and  Panikkar MVN, 2010. Evaluation of seaweeds bioactives on common aquatic floral and faunal weeds of shrimp ponds.Thalassas.27(1): 47-56.
Meena R,  Chaudhary J,P  Agarwal PK,  Maiti P,  Chatterjee S,  Raval HD,  Agarwal P,  Siddhanta AK,  Prasad K and   Ghosh PK, 2014. Surfactent - induced coagulation of agarose from Aqueous extract of Gracilaria dura seaweed as an energy-efficient alternative to the conventional freeze-thaw process. Royal society of chemistry 4: 28093-28098.
Mogle PU and Maske RS, 2012. Effect of some plant extracts aganist seed borne infection of Collectotricumdestructivum on Vingauniguculata L. Bioscience Discovery. 3(2): 266-269.
Musharraf SG,  Ahmed MA,  Zehra N,  Kabir N,  Choudhary MI and Rahman  AU, 2012. Biodiesel production from micro algal isolates of southern Pakistan and quantification of FAMEs by GC-MS/MS analysis. Chem. Cent. J. 6(1): 149.
Narayanamoorthi V,  Vasantha K,  Rency RC and Maruthasalam A, 2015. GCMS determination of bioactive components of Pepromia pellucida (L.) Kunth. Bioscience Discovery, 6(2): 83-88.
Oh KB,  Lee JH,  Chung SC,  Shin J,  Shin HJ,  Kim HK  and Lee HS, 2008. Antimicrobial activities of the bromophenols from the red alga Odonthalia corymbifera and some synthetic derivatives. Bioorg. Med. Chem. Lett. 18: 104.
Pandurangan A, Perisamy M, Sekaranm S, Jebamalaim SK and Sarangam B, 2010. Pharmacognostical and antifungal activity of selected seaweeds from Gulf of Mannar region.Recent Research in Science and Technology, 2(1): 115-119.
Smit AJ, 2004. Medicinal and pharmaceutical uses of seaweed natural products: a review. Journal Applied Phycology. 16: 245-262.
Siddhanta AK,  Meena R,  Prasad K,  Ramavat BK,  Ghosh PK,  Eswaran K,  Thiruppathi  S and Mantri VA, 2005. A cost effective process for preparing agarose from Gracilaria spp. PCT Patent Publication No. W0 2005/118830 A1.
Tabarsa M,  Rezaei M,  Ramezanpour Z and  Waaland JR,  2012. Chemical compositions of the marine algae Gracilariasalicornia (Rhodophyt) and Ulvalactuca (Cholorophyta) as a potential food source.J Sci. Agric. 92: 2500-2506.
Usha R and Maria Victoria Rani S, 2015. Gas chromatography and mass spectrometric analysis of Padina pavonia (L.) Lamour. Bioscience Discovery, 6(1): 01-05.
Veeragurunathan  V,  Eswaran  K,  Saminathan,  KR  Vaibhav  AM,  Malarvizhi  J,   Ajay G and   Jha B, 2015.  Feasibility of Gracilaria dura cultivation in the open sea on the Southeastern coast of India. Aquaculture. 438:68-74.
 Wen X,  Peng C,  Zhou H,  Lin Z,  Lin G,  Chen S and  Li  P, 2006. Nutritional composition and assessment of Gracilaria lemaneiformis Bory.J. Integr. Plant Biol. 48: 1047-1053.

How to Cite this Article:
Biji Cyriac and K. Eswaran, 2015. GC - MS determination of bioactive components of Gracilaria dura (C.Agardh) J. Agardh. Science Research Reporter, 5(2):100-105.

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