Current Issue Volume 5, Number 2 (Oct - 2015) ...
Latest Updates
Current Issue of Bioscience Discovery Published
Volume 6, No. 1, Jan. 2015
Quick Links:
Bioscience Discovery
An International Journal of Life Sciences
Available on http://biosciencediscovery.com
More

A Computational Approach for Structural Analysis of Bap, a Biofilm Formation Protein in Staphylococcus aureus 

Gajanan Kashinathrao Arbade*

Department of Biosciences and Technology,
Defence Institute of Advanced Technology, Girinagar, Pune- 411025, MS, India.
*gajananarbade@gmail.com

Article Info

 

Abstract

Received: 27-04-2015,
Revised: 18-06-2015,
Accepted: 26-07-2015

In the present study, we have analyzed the residual information for the better explanation of properties of Bap protein based on in-silico approaches. The main objective of this study to get insight into the structural aspects of the Bap protein. This approach could gainfully be applied for the design of an efficient anti staphylococcal compounds to solve both the problems, health issues in human and other animals as well as contamination problems in the variety of medical instruments such as catheters. This work involves the study of physicochemical properties of the Bap protein. We have predicted secondary structure of the Bap protein and generated 3D structure of Bap protein by using homology modeling approach.

Keywords:
Adhesive   property, Bap, Biofilm, Homology modeling, Staphylococcus aureus.

FULL TEXT (Click here)

REFERENCES

Amorena BE, Gracia E, Monzón M, Leiva J, Oteiza C, Pérez M, Alabart JL and Hernández YJ, 1999. Antibiotic susceptibility assay for Staphylococcus aureus in biofilms developed in vitro. J. Antimicrob. Chemother. , 44:43–55.
Ansari SN and lliyos S, 2011. A comparative study of protein structure visualization tools for various display capabilities. Bioscience Discovery 02 (2):222 -226.
Arbade GK and Srivastava SK, 2015. Cloning, expression, purification, crystallization and preliminary X-ray diffraction studies of NAD Synthetase from methicillin-resistant Staphylococcus aureus. Acta. Cryst. F71, 763-769.
Berman HM, Westbrook J, Feng Z, Gilliland G, Bhat TN, Weissig H, Shindyalov IN  and Bourne PE, 2000. The Protein Data Bank. Nucleic acids res., 28(1):235-242.
Chou PY, and Fasman GD, 1974. Conformational parameters for amino acids in helical, β-sheet, and random coil regions calculated from proteins. Biochemistry. , 13(2): 211–222.
Costerton JW, Lewandowski Z, Caldwell DE, Korber DR and Lappin-Scott HM, 1995. Microbial biofilms. Annu. Rev. Microbiol., 49:711–745.
Cucarella C Tormo MA, Ubeda C, Trotonda MP, Monzon M, Peris C, Amorena B, Lasa I and  Penades JR, 2004. Role of Biofilm-Associated Protein Bap in the Pathogenesis of Bovine Staphylococcus aureus. Infect. Immun., 72 (4): 2177-2185.
Cucarella C, Solano C, Valle J, Beatriz Amorena B, Lasa I, Penades AR, 2001. Bap, a Staphylococcus aureus Surface Protein Involved in Biofilm Formation. J. Bacteriol., 83(9):2888-2896.
Donlan RM, 2001. Biofilms and Device-Associated Infections, Emerging Infectious Diseases, 7(2) 278-281.
Donlan RM, 2000. Role of biofilms in antimicrobial resistance. ASAIO J., 46:S47–S52.
Flemming H and Wingender J, 2010. The biofilm matrix. Nat Rev Microbiol., 8: 2888-2896.
Gasteiger E, Hoogland C, Gattiker A, Duvaud S, Wilkins MR, Appel RD, Bairoch A and Walker JM,2005. Protein Analysis Tools on the ExPASy Server. The Proteomics Protocols Handbook.  571-607.
Kodgire S, Pawar V, Wagh N, Kamble L and Zore G, 2015. In  Silico  Analysis  of  HMG  CO-A  Reductase  of  Candida  albicans SC5314. Science Research Reporter, 5(1):01-08.
Lovell SC Davis IW, Arendall WB , de Bakker PI, Word JM, Prisant MG, Richardson JS and Richardson DC, 2002. Structure validation by C-alpha geometry: phi, psi and C-beta deviation. Proteins: Structure, Function & Genetics., 50: 437-450. 
Monzon MC Oteiza C, Leiva J, Lamata M and Amorena B, 2002. Biofilm testing of Staphylococcus epidermidis clinical isolates: low performance of vancomycin in relation to other antibiotics. Diagn. Microbiol. Infect. Dis., 44:319–324.
Munduganore DS Mundaganore YD and Ashokan KV, 2012; Sequence analysis of protein in peste des petits ruminants: an in silico approach. JFAV. , 2 (3):26-36.
Newman JA, Rodrigues C and Lewis RJ, 2013. Molecular Basis of the Activity of SinR, the Master Regulator of Biofilm Formation in Bacillus SubtilisJ. Biol. Chem. 288: 10766.
Schwede T Kopp J, Guex N and Peitsch MC, 2003. SWISS-MODEL: an automated protein homology-modeling server. Nucleic Acids Res., 31(13):3381–3385.
Thakare PV Chaudhari US, Makhe MS, Deshmukh VP and Kurtkoti RR, 2010. Secondary Structure Prediction and Phylogenetic Analysis of Salt Tolerant Proteins, Global J. Mol. Sci., 5 (1): 30-36. 
Valle J Latasa C, Gil C, Toledo-Arana A, Solano C, Penades JR and Lasa I, 2012. Bap, a Biofilm Matrix Protein of Staphylococcus aureus Prevents Cellular Internalization through Binding to GP96 Host Receptor. PLOS pathogen. , 8 (8): e1002843.
Wertheim HF Vos MC, Ott A, van Belkum A, Voss A, Kluytmans JA, van Keulen PH, Vandenbroucke-Grauls CM, Meester MH and Verbrugh HA, 2004. Risk and outcome of nosocomial Staphylococcus aureus bacteraemia in nasal carriers versus non-carriers. Lancet. , 364, 703-705.

How to Cite this Article:
Gajanan Kashinathrao Arbade, 2015. GC - A Computational Approach for Structural Analysis of Bap, a Biofilm Formation Protein in Staphylococcus aureus. Science Research Reporter, 5(2):112-117.

Editor: Dr. Umesh P. Mogle, Printed, Published and Owned by Dr. Umesh P. Mogle, Published from ‘Kaushalyaaie’, P. No. 43, Priyanka Residancy, Mantha Chaufulli Jalna 431203 (M.S.) India.


Quick Link to Bioscience Discovery

Bioscience Discovery home
Archives
Instructions to Author
Subscription
Submission of Research Paper Current Issue

Photo Gallery

Follow Us (Social Sites)