Screening, Characterization and Biosynthesis of Cellulase from Trichoderma viride on rice bran under solid state fermentation

Nadagouda M.G1, Lingappa K2  Malipatil S.G1 and V. S. Bheemareddy3

1Dept. of Botany.  Smt. ASM College for women’s, Bellary – 583102,
2Department of Microbiology, Gulbarga university, Gulbarga. India.
3Department of Botany, J. S. S. College, Dharwad, India-04
*Corresponding author e-mail: nadagoudmg@gmail.com

Article Info		Abstract
Received: 04-08-2015, Revised: 01-09-2015, Accepted: 05-09-2015		Although a large number of microorganisms are capable of degrading cellulose, only a few of these microorganisms produce significant quantities of cell-free cellulase enzymes capable of completely hydrolyzing crystalline cellulose in vitro.    In the present investigations nearly 25 different strains of  Trichoderma viride on CMC agar media were isolated and identified and all are having very good capacity to degrade Cellulose enriched media based on their enzymatic properties and clear zone appears around the each colony. The Cellulase synthesis was screened by employing different substrate - rice bran, wheat bran, cotton waste, ground nut husk and saw dust by fermentation parameters. The supplement of lactose and corn-steep solid to the media e favored the enzyme formation markedly. The nitrogen sources such as corn steep solid and Ammonium nitrate enhanced the production of cellulase enzyme.
Keywords: Cellulase,  rice bran, Ammonium nitrate,  wheat bran, cotton waste, ground nut husk, Trichoderma viride

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How to Cite this Article:
Nadagouda MG, Lingappa K  Malipatil SG and VS Bheemareddy, 2015. Screening, Characterization and Biosynthesis of Cellulase from Trichoderma viride on rice bran under solid state fermentation. Science Research Reporter, 5(2): 177-180.

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