Current Issue Volume 5, Number 2 (Oct - 2015) ...
Latest Updates
Current Issue of Bioscience Discovery Published
Volume 6, No. 1, Jan. 2015
Quick Links:
Bioscience Discovery
An International Journal of Life Sciences
Available on

Screening, Characterization and Biosynthesis of Cellulase from Trichoderma viride on rice bran under solid state fermentation

Nadagouda M.G1, Lingappa K2  Malipatil S.G1 and V. S. Bheemareddy3

1Dept. of Botany.  Smt. ASM College for women’s, Bellary – 583102,
2Department of Microbiology, Gulbarga university, Gulbarga. India.
3Department of Botany, J. S. S. College, Dharwad, India-04
*Corresponding author e-mail:

Article Info



Received: 04-08-2015,
Revised: 01-09-2015,
Accepted: 05-09-2015

Although a large number of microorganisms are capable of degrading cellulose, only a few of these microorganisms produce significant quantities of cell-free cellulase enzymes capable of completely hydrolyzing crystalline cellulose in vitro.    In the present investigations nearly 25 different strains of  Trichoderma viride on CMC agar media were isolated and identified and all are having very good capacity to degrade Cellulose enriched media based on their enzymatic properties and clear zone appears around the each colony. The Cellulase synthesis was screened by employing different substrate - rice bran, wheat bran, cotton waste, ground nut husk and saw dust by fermentation parameters. The supplement of lactose and corn-steep solid to the media e favored the enzyme formation markedly. The nitrogen sources such as corn steep solid and Ammonium nitrate enhanced the production of cellulase enzyme.  

Cellulase,  rice bran, Ammonium nitrate,  wheat bran, cotton waste, ground nut husk, Trichoderma viride

FULL TEXT (Click here)

Beatriz G, Remedios Y, Alonso JL and Parajo JC, 2008. L-Lactic acid production from apple pomace by sequential hydrolysis and fermentation. Bioresource Technol., 99:  308–319.
Cen PL and Xia LM, 1999 Production of cellulase by solid-state fermentation, Adv. Biochem. Engin. Biotechnol., 65 :  68– 92.
Han SO, Yukawa H,  Inui M, and Doi RH, 2003. Regulation of expression of cellulosomal cellulase and hemicellulase genes in Clostridium cellulovorans. J. Bacteriol., 185: 6067-6075.
Himmel   ME,    Ruth   MF,    Wyman   CE,  1999.  Cellulase for commodity products from cellulosic biomass.  Curr. Opin. Biotechnol.,10: 358- 364 Kasing Apun, Sarawak., 1995. Cellulase  production. Practical Biotechnology,  National Centre for Biotechnology Education, Malaysia.
Parani K and Eyini M, 2012. Production of ligninolytic enzymes during solid state fermentation of coffee pulp by selected fungi. Science Research Reporter 2(3):202-209.
Rauscher  RE,   Wurleitner  C, Wacenovsky N,  Aro  AR,  Stricker  S,  Zelinger CP, Kubicek  M,  Penttila  and Mach RL,  2006. Trancriptinal regualtion fo xyn1, encoding xylansse 1 in Hypocrea jecorina. Eukaroytic Cell., 5: 447: 456.
Khan Rehan Saleem and Zia H Khan, 2014. Studies on Chitinase isolation and thermostability between Bacillus Circulance Strain L2 and Bacillus Licheniformis Strain 2J. Science Research Reporter 4 (1):01-07.
Saleem F, Ahmed S  and Jamil A, 2008. Isolation of a xylan degrading gene from genomic DNA library of a thermophilic fungus Chaetomium thermophile ATCC 28076. Pak. J. Bot., 40: 1225-1230.
Sa-Pereira  PH,  Paveia M,  Costa-Ferreira and Aires-Barros MR,  2003. A new look at xylanases: An overview of purification strategies. Mol. Biotechnol., 24: 257-281.
Seiboth B, Pakdaman BS, Hartl L and Kubicek CP 2007. Lactose metabolism in filamentous fungi: how to deal with an unknown substrate. Fungal Biology Reviews, .21:42-48.
Sun T, Liu BH, Li ZH and Liu DM, 1999.  Effects of air pressure amplitude on cellulase productivity by Trichoderma viride SL-l in periodic pressure solid state fermenter, Process Biochem., 34:25–29.
Yang CH, Yang SF and  Liu WH, 2007. Produciton of xylo oligosaccharfides from xylans by extracellular xylanases from Thermobida fusca. J.  Agri. Food Chem., 55:3955-3959.

How to Cite this Article:
Nadagouda MG, Lingappa K  Malipatil SG and VS Bheemareddy, 2015. Screening, Characterization and Biosynthesis of Cellulase from Trichoderma viride on rice bran under solid state fermentation. Science Research Reporter, 5(2): 177-180.

Editor: Dr. Umesh P. Mogle, Printed, Published and Owned by Dr. Umesh P. Mogle, Published from ‘Kaushalyaaie’, P. No. 43, Priyanka Residancy, Mantha Chaufulli Jalna 431203 (M.S.) India.

Quick Link to Bioscience Discovery

Bioscience Discovery home
Instructions to Author
Submission of Research Paper Current Issue

Photo Gallery

Follow Us (Social Sites)