In vitro regeneration of Momordicadioica Roxb. through leaf derived callus

Swamy T N1 Bylla Prasad2 V Suresh1 and Md. Mustafa1

Plant Tissue culture and Molecular Taxonomy laboratory Department of Botany1, Department of Biotechnology2, Kakatiya University Warangal 506009 Telangana state India.
*mustafarz76@gmail.com

Article Info		Abstract
Received: 01-05-2015, Revised: 02-08-2015, Accepted: 17-08-2015		The present work describes an efficient protocol for in vitro regeneration in  Momordica dioca with leaf explants. The leaf explants were inoculated on MS medium supplemented with different combinations and concentration of 3.0mg/l BAP+0.5mg/l NAA. After 3 weeks of cultures were observed proliferated callus this callus was turned to white friable and green compact callus in combination with high concentration with cytokinin (1 mg/l BAP). After sub culture of same callus in 3.0mg/l BAP+0.5mg/l NAA concentration were observed that 80% of regenerated plantlet was found. With low concentration of cytokinin (0.5 BAP) no callus induction were observed.
Keywords: In vitro regeneration leaf derived callus BAPNAA

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References
Ali MOH Fuji T and Fujieda K, 1991. Techniques of propagation and breeding of Kakrol (Momordicadioica Roxb.). Sci. Horticul. 47: 335-338.
Bharathi LK Naik G Singh HS and  Dora DK, 2007. Spine Gourd. In: Peter KV (ed) Underutilized and Underexploited Horticultural Crops. New. India Publishing New Delhi pp. 289-295.
Bhattacharya Salil K Sen Parantapa Ray Arunabha, 2004. Motor disorders In: Das Prasun K. (ed.) Pharmacology. 2nd Ed. New Delhi: Elsevier 247-25.
BhavanaBawara Mukesh Dixit NS ChauhanVK Dixit DK Saraf, 2010. International Journal of Phytomedicine.2 :01-09.
Deokule SS, 2006. Ethnobotany plants of Baramati region of Pune District of Maharastra. J. Ethnomed. Taxon. Bot. 30: 59-69.
Dronne S JullienFCaissard J C and Faure O, 1999. A simple and efficient method for in vitro shoot regeneration from leaves of lavandin (LavandulaxintermediaEmeri ex Loiseleur). Plant Cell Rep., 18:429 – 433.
Halder T and Gadgil VN, 1982.  Shoot bud differentiation in long term callus cultures of Momordica and Cucumis Ind. J. Exptl. Biol., 22:780-782.
HoqueA Islam R and Arima S, 2000.High frequency plant regeneration from cotyledon derived callus of Momordicadioica (Roxb) Wild. Phytomorphology,  50:267 – 272.
Jain A Soni M and  Deb L, 2008. Antioxidant and hepatoprotective activity of ethanol and aqueous extracts of MomordicadioicaRoxb. Leaves. J. Ethnopharmacol., 4: 115-118.
Kumar P P Rao C D and Goh C J, 1998. Influence of petiole and lamina on adventitious   shoot initiation from leaf explants of Paulownia fortune. Plant Cell  Rep., 17:886 – 890.
Luo L Li Z Zhang Y and  Huang R, 1998. Triterpenes and steroidal compounds from Momordicadioica. Yao-Xue-Xue-Bao. 33: 839-42.
Maharana T and  Tripathy P, 1996. Agro techniques for growing spine gourd in pots. Indian Hortic (Jan. Mar.):16–17.
Nabi SA Rashid MM Al-Amin M and Rasul MG, 2002. Organogenesis in teasle gourd (MomordicadioicaRoxb.). Plant Tiss. Cult., 12: 173-180.
Nerurkar LM, Johns LM, Buesa, 2011. Momordica charantia (bitter melon) attenuates high-fat diet-associated oxidative stress and neuroinflammation J. Neuroinflamm., 8:1–19
Nyochemdeng LM and Garton  S, 1998. Plant regeneration form cocoyam callus derived from shoot tip and petioles. Plant .Cell. Tiss. Org. Cult., 53:127-134.
Rakh MS and  Chaudhari SR, 2010. Literature survey of plant MomordicadioicaRoxb. Willd. An update. Int. J. Pharma. Res. Dev.1-8.
Ram  D Banerjee M K Pandey  S and  Srivastava  U, 2001. Collection and evaluation of kartoli (MomordicadioicaRoxb. Ex. Wild.). Indian Journal of Plant Genetic Resources,14: 114-116.
Rasul M G Hiramatsu M and  Okubo H, 2007. Genetic relatedness (diversity) and cultivar identification by randomly amplified polymorphic DNA (RAPD) markers in teasle gourd (MomordicadioicaRoxb.). Sci. Horticult., 111: 271-279.
Reddy G Ravi K B Krishna M G and  Mullangi R, 2006. Anity per glycolic activity of Momordicadioica fruits in alloxan-induced diabetic rats. Asian J. Pharmacodyn. Pharmacokine,. 6: 327-329.
Rey HY ScocchiA M and Gonzaez AM, 2000. Plant regeneration in Arachispintoi (Leguminosae) through leaf culture. Plant Cell Rep., 19:856 -862.
Saima M Muhammad Z Riaz-ur-Rehaman MF and  Chaudhary, 2007. In vitro regeneration from Direct and Indirect organogenesis of Momordicacharantia. Pakistan J. Bio. Sci., 10(22):4118-4122.
Singh MN, Kathal R and Bhatnagar SP, 1990. Regeneration of plants from hypocotyl and cotyledon cultures of CucumismelocvpusaMaduras. Phytomorphology, 40: 401-405.
Singh PK Singh AK Singh HM Kumar P and  Yadav CB, 2009. Insect-pests of spine gourd (MomordicadioicaRoxb.) and efficacy of some insecticides against the epilating beetle Henosepilachnavigintioctopunctatu(F.) a serious pest of this Crop. Pest Management .Econ. Zool.,17:85–91.
Trivedi RN & Roy RP, 1972. Cytological studies in some species of Momordica. Gentica., 43: 282-291.
Yokozawa HY, Kim HJ Kim, 2007. Amla (Emblica officinalis Gaertn.) prevents dyslipidaemia and oxidative stress in the ageing process Br. J. Nutr., 97:1187–1195.          

How to Cite this Article:
Swamy TN Bylla Prasad V.Suresh and Md.Mustafa, 2015. In vitro regeneration of Momordicadioica Roxb. through leaf derived callus. Science Research Reporter, 5(2): 177-180.

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