| Current Issue | Volume 6, Number 1 (April - 2016) ... |
Hydrocarbon degradation potential of indigenous fungal isolates from Indian oil refinery, Haldia, (W. B.) India
Shreyasri Dutta* and Padma Singh
Microbiology Department, Kanya Gurukul Campus, Gurukul Kangri University, Haridwar-249407
*Email: shreyasridutta@gmail.com
Article Info |
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Abstract |
Received: 22-02-2016, |
Application of fungus for effective removal of hydrocarbon contamination from soil has been considered as an effective way. In present study contaminated soil samples from three sites of Indian oil refinery, Haldia, W.B were studied for assessment of their refine oil degrading potential. The average refines oil utilizing fungi varied from 4.13X102+0.88CFU/g to 3X102+0.61CFU/g. Nine fungal isolates recovered from oil-contaminated soils were screened for refine oil biodegradation activity in a shake-flask culture by using 2, 6-DCPIP as a indicator. Among the nine fungal isolates, only five showed potential for biodegradation. The identified fungal genera included Aspergillus flavus, Aspergillus terrus, Aspergillus niger, Penicillium sp. and Gleocladium sp. Biodegradation ability of all isolates was confirmed by vapour phase transfer and CO2 evolution method. The results showed that indigenous fungal isolates displayed highest capability for biodegradation of refine petroleum oil. Hence, these fungal species can be used for bioremediation of petroleum contaminated sites. |
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REFERENCES
Alvarez PJJ, Anid PJ, Vogel TM, 1991. Kinetics of aerobic biodegradation of benzene and toluene in sandy aquifer material. Biodegradation, 2:43-51.
April TM, Foght JM, Currah RS, 2000. Hydrocarbon degradation filamentous fungi isolated from flare pit soils in Northern and Western Canada. Can. J. Microb., 46:38-49.
ASTM D 5864. 1995. In the U.S., the ASTM committee adopted the OECD 301-B Modified Sturm procedures within ASTM D-5864-00 Standard Test Method for Determining Aerobic Aquatic Biodegradation of Lubricants (originally published in 1995)
Autry AR and Ellis G M, 1992. An effective remedial alternative for petroleum hydrocarbon contaminated soil. Bioremediation, 11 (4):318-323.
Barnett HL, 2003. Manual for Hypomycetes fungi. The APS. St. Paul, Mnnesota, 55121-2097, USA.
Bouchez M, Blanchet D, Haeseler F, Vandecasteede, 1996. Les hydrocarbures aromatiques polycycliques dans l’environnement. Revue de l’Institut français du pétrole, 51:797-828.
Chaudhry S, Luhach J, Sharma V, Sharma C, 2012. Assessment of diesel degradation potential of fungal isolates from sludge contaminated soil of petroleum refinery, Haryan Research journal of microbiology, 7(3):182-190.
Dutta S, Singh P, 2014a. The Plackett-Burman Model-Optimization of Significant Nutritional Parameters for Petroleum Bioremediation by Pseudomonas sp, International Journal of Advanced Research, 2:898-902.
Dutta S, Singh P, 2014b. Laboratory Scale Experiment on the Bioremediation of Refined Petroleum Hydrocarbon by using CO2 Evolution Method. International Journal of Basic and
Applied Biology, 1: 6-10
EPA-560/6-82-003, PB82-233008, 1982. Test guidelines: chemical fate -- aerobic aquatic biodegradation.
Hanson, Desai KG, Desai JD, 1993. A rapid and simple screening technique for potential crude oil degrading microorganisms. Biotechnology Techniques, 7:745-748.
Juhasz, AL, Naidu R, 2000. Bioremediation of high molecular weight polycyclic aromatic hydrocarbons: a review of the microbial degradation of benzo[a]pyrene. Intern Biodeterioration&Biodegradation, 45:57-88.
Lemos JLS, Rizzo ACL, Millioli V, Santos R, 2002. Petroleum degradation by filamentous fungi. Environmental Conferences - IPEC - The University of Tulsa. Publishing in Uni of Tulsa.
Lotfinasabasl S, Gunale VR, Rajurkar NS, 2012. Assessment of petroleum hydrocarbon degradation from soil and tarball by fungi. Bioscience Discovery, 3(2): 186-192.
Mariano AP, Kataoka APAG, Angelis DFA, Bonotto DM, 2007. Laboratory study on the bioremediation of Diesel oil contaminated soil from petrol station. Brazilian J. of Microbio, 38:346-353.
Maygaonkar PA, Wagh PM, Permeswaran U, 2012. Biodegradation of distillery effluent by fungi. Bioscience Discovery 3(2): 251 -258.
Mills AL, Breuil C, Colwell RR, 1978. Enumeration of petroleum degradation marine and Estuarine microorganisms by the most probable Number methods. Can J Microbiol, 24:552 557.
Mittal A and Singh P, 2009. Studies on biodegradation of crude oil by Aspergillus niger. The south pacific Journal of Natural Science, 27:57-60.
Ojumu TV, Bello OO, Sonibera JA, Solomon BO, 2004. Evaluation of microbial systems for bioremediation of petroleum refinery effluents in Nigeria. Afr J Biotechnol, 4:31-35.
Okpokwasili GC, Amanchukwu SC, 1988. Petroleum hydrocarbons degradation by Candida spp. Environ. Int., 14:243-247.
Propst TL, Lochmiller RL, Qualls CW, McBeek, 1999. In situ (mesocosm) assessment of immunotoxicity risks to small mammals inhabiting petrochemical waste sites. Chemosphere, 38: 1049-1067.
Santos HF, Camero A, Paes JES, Rosado S, Peixoto RS, 2011. Bioremediation of Mangroves Impacted by Petroleum. Water Air Soil poll, 216: 329-350.
Thijsse GJ, Avan der Linden, 1961. Iso alkane oxidation by a Pseudomonas. Antonie Van leeuwenhoek, 27:171-179.
Waksman SA, Fred EB, 1922. A tentative outline of the plate method for determining the number of microorganisms in the soil. Soil Sci., 14:27-28.
Westlake DW, Jobson A, Phillippe R, Cook FD, 1974. Biodegradability and crude oil composition. Can J Microbiol, 20(7):915-928.
Yateem A, 1998. White rot fungi and their role in remediating oil-contaminated soil. Environment International, 24:181-187.
Zhang XCL, Peterson D, Reese G, Möller & R. Haws, 1995. Biodegradation of Biodiesel I the aquatic environment. Department of Agricultural Engineering, University of Idano, Moscow, Indano, USA.
Shreyasri Dutta and Padma Singh, 2016. Hydrocarbon degradation potential of indigenous fungal isolates from Indian oil refinery, Haldia, (W. B.) India. Science Research Reporter, 6(1):04-11.
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