Current Issue | Volume 6, Number 2 (October - 2016) ... |
In-vitro antimicrobial activity of lichen Ramalina conduplicans Vain. collected from Eastern Ghats, India
Anjali Devi B.1, Satish Mohabe1, Sanjeeva Nayaka2 and A. Madhusudhana Reddy1*
1 Lichenology Laboratory, Department of Botany, Yogi Vemana University, Vemanapuram, Kadapa - 516003, Andhra Pradesh, India
2Lichenology Laboratory, CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow - 226 001, Uttar Pradesh, India
*grassced@gmail.com
Article Info |
|
Abstract |
Received: 02-08-2016 |
The research work investigates the in-vitro antimicrobial efficacy of lichen Ramalina conduplicans Vain. (Ramalinaceae). The 2-Propanol, methanol, acetone and petroleum ether extract of the species were tested each against eleven bacterial and fungal pathogens by using Kirby-Bauer disc diffusion method with 15 μL extract per disc. It is observed that the extracts are effective against bacteria in than to the fungi. Among various extracts methanol was much effective against most of the bacteria and diameter of inhibition zone ranged from 12.66±0.66 to 16.66±1.6 mm. In case of antifungal activity the extract formed zone of inhibition against only six pathogens. The R. conduplicans can be a potential source of bioactive agents against disease causing microbes of humans and plants. |
|
Keywords: |
REFERENCES
Acharius E, 1810. Lichenographia Universalis. Göttingae. 122: 598.
Anjali DB, Mohabe S, Reddy MA, Nayaka S, Ponmurugan P and Ayyappadasan G, 2014. Antimicrobial Activity of Lichen Roccella montagnei Bél. obtained from Horsley hills, Andhra Pradesh, India. In: Madhusudhana Rao J. (Eds). Bioactives from Natural Products, Special Issue (pp13–20), Proceedings of Andhra Pradesh Academy of Sciences.
Anjali DB, Mohabe S, Reddy AM and Nayaka S, 2015a. Efficacy of a potential lichen Parmotrema andinum (Müll. Arg.) Hale against pathogenic microorganisms. Journal on New Biological Reports, 2(4): 149–156.
Anjali DB, Mohabe S, Reddy MA and Nayaka S, 2015b. Antimicrobial activities of 2-Propanol crude extract from lichen Parmotrema tinctorum (Despr. ex. Nyl.) Hale, collected from Eastern Ghats, India. Curr. Res. Environ. Appl. Mycol. J. Fungal Biol.,5 (3): 160–168.
Awasthi DD, 2007. A compendium of the Macrolichens from India, Nepal and Sri Lanka. Bishen Singh Mahendra Pal Singh, Dehra Dun.
Behera BC, Verma N, Sonone A and Makhija U, 2009. Optimization of culture conditions for lichen Usnea ghattensis G. Awasthi to increase biomass and antioxidant metabolite production. Food Technol. Biotech., 47(1): 7–12.
Bernasconi ES, De Vito IE, Martinez LD and Raba J, 2000. Heavy metals determination by ICP-AES coupled with ultrasonic nebulization using the lichen Usnea densirostra (Tayl.) as biomonitor pollution in San Luis, Argentina. Ars Pharmaceutical, 41(3): 249-257.
Bhattarai T, Subba D and Subba R, 1999. Nutritional value of some edible lichens of east Nepal. Journal of Applied Botany and Food Quality – Angewandte Botanik, 73:11–18.
Canarasan D, Kahya D, Yurdakulol E and Atakol O, 2006. Identification and quantitation of Usnic acid from the lichen Usnea species of Anatolia and antimicrobial activity. Z. Naturforsch., 61c: 773–776.
Candan M, Yilmaz M, Tay T, Kivanc M and Turk H, 2006. Antimicrobial activity of extracts of the lichen Xanthoparmelia pokornyi and its Gyrophoric and Stenosporic acid constituents. Z. Naturforsch., 61c: 319-323.
Harwood LM and Moody CJ, 1989. Experimental organic chemistry: Principles and Practice (Illustrated Edn.), Blackwell Scientific Publications, 122–125.
Kambar Y, Vivek MN, Manasa M, Kekuda TRP and Ramesh Kumar KA, 2014. Proximate and elemental analysis of Ramalina conduplicans Vain. (Ramalinaceae) and Parmotrema tinctorum (Nyl.) Hale (Parmeliaceae). J. Chem. Pharm. Res., 6(6): 2668–2674.
Kekuda TRP, Vinayaka KS, Swathi D, Suchitha Y, Venugopal TM and Mallikarjun N, 2011. Mineral composition, total phenol content and antioxidant activity of a macrolichen Everniastrum cirrhatum (Fr.) Hale (Parmeliaceae). E- J. Chem.,8(4): 1886-1894.
Kowalski M, Hausner G and Piercey-Normore MD, 2011. Bioactivity of secondary metabolites and thallus extracts from lichen fungi. Mycoscience, 52: 413–418.
Kumar PSV, Kekuda PTR, Vinayaka, KS, Swathi D and Chinmaya A, 2010. Insecticidal efficacy of Ramalina hossei H. Magn and G. Awasthi and Ramalina conduplicans Vain- Macrolichens from Bhadra wildlife sanctuary, Karnataka. Biomedicine, 30(1): 100–102.
Lawrey JD, 1986. Biological role of lichens substances. The Bryologist, 89: 111–122.
Luo H, Wei X, Yamamoto Y, Liu Y, Wang L, Jung JS, Koh YJ and Hur J, 2010. Antioxidant activities of edible lichen Ramalina conduplicans and its free radical-scavenging constituents. Mycoscience, 51(5): 391–395.
Mohabe S., Anjali D. B., Reddy A.M., Nayaka S. and Chandramati P. S. (2015c). An appraisal of lichen biota in Chittoor district of Andhra Pradesh, India. In (Ed.) Biodiversity in India, T. Pulaiah & Sandhya Rani (8): 247–296.
Nash TH, 1996. Lichen Biology. 1st Edn. Cambridge University Press, Cambridge.
Nayaka S, Upreti DK and Khare R, 2010. Medicinal lichens of India. In PC Trivedi (Ed.), Drugs from Plant. Avishkhar Publishers, Distributors, Jaipur, India.
Orange A, James PW and White FJ, 2001. Microchemical methods for the identification of lichens. British Lichen Society, U.K.
Palo TR, 1993. Usnic acid, a secondary metabolite of lichens and its effect on in vitro digestibility in reindeer. Rangifer, 13(1): 39–43.
Pavithra GM, Vinayaka KS, Rakesh KN, Syed J, Dileep N, Prashith Kekuda TR, Saba S, Abhishiktha and S Naik, 2013. Antimicrobial and antioxidant activities of a macrolichen Usnea pictoides G. Awasthi (Parmeliaceae). Journal of Applied Pharmaceutical Science, 3 (08); 154–160.
Prabhu SS and Sudha SS, 2015. Evaluation of the antibacterial properties of some Lichen species against human pathogens. Int. J. Adv. Res. Biol. Sci. 2(4): 177–181.
Preeti S Babiah, Upreti DK and John SA, 2014. Fungicidal Efficacy of a Foliose Lichen Flavoparmelia caperata (L.) Hale against Phytopathogenic Fungi. Int. J. Curr. Res. Biosci. Plant Biol., 1(5): 38–44.
Ribeiro-Costa RM, Alves AJ, Santos NP, Nascimento SC, Goncalves ECP, Silva NH, Honda NK, and Santos-Magalhaes NS, 2004. In vitro and in vivo properties of usnic acid encapsulated into PLGA-microspheres. J. Microencapsulation , 21(4): 371–384.
Sati SC and Joshi S, 2011. Antibacterial Activity of the Himalayan Lichen Parmotrema nilgherrense Extracts. Br. Microbiol. Res. J., 1(2): 26–32.
Shukla P, Upreti DK and Tiwari P, 2014. Assessment of dye yielding potential of Indian lichens. Ind. J. Pl. Sc., 3(1): 57–63.
Soxhlet F, 1879. Die Gewichtsanalytische Bestimmung des Milchfettes. Dingler’s polytechnisches J., 232: 461–465.
Temina M, Levitsky DO and Dembitsky VM, 2010. Chemical constituents of the epiphytic and lithophilic lichens of the genus Collema. Rec. Nat. Prod., 4(1): 79–86.
Turk H, Yilmaz M, Tay T, Turk AO and Kivanc M, 2006. Antimicrobial activity of extracts of chemical races of the lichen Pseudevernia furfuracea and their Physodic Acid, Chloroatranorin, Atranorin, and Olivetoric acid constituents. Z. Naturforsch., 61c: 499-507.
Upreti DK, Divakar PK and Nayaka S, 2005. Commercial and ethnic use of lichens in India. Econ. Bot., 59: 269–273.
Verma N, Behera BC and Sharma BO, 2012. Glucosidase inhibitory and radical scavenging properties of lichen metabolites Salazinic Acid, Sekikaic Acid and Usnic Acid. Hacettepe J. Biol. & Chem., 40(1): 7–21.
Vinayaka KS, Kumar PSV, Kekuda PTR, Krishnamurthy YL, Mallikarjun N and Swathi D, 2009. Proximate composition, antioxidant, anthelmintic and insecticidal activity of a macrolichen Ramalina conduplicans Vain. (Ramalinaceae). Europ. J. Appl. Sci., 1(3): 40–46.
Wei X, Jeon H, Han KS, Koh YJ and Hur J, 2008. Antifungal activity of lichen forming fungi against Colletotrichum acutatum on hot pepper. Plant Pathol. J., 24(2): 202–206.
Yilmaz M, Turk AO, Tay T and Kivanc M, 2004. The antimicrobial activity of extracts of the lichen Cladonia foliacea and its (-)-Usnic acid, Atranorin, and Fumar protocetraric acid constituents. Z. Naturforsch., 59c: 249–254.
How to Cite this Article:
Anjali Devi B, Satish Mohabe, Sanjeeva Nayaka and A. Madhusudhana Reddy, 2016. In-vitro antimicrobial activity of lichen Ramalina conduplicans Vain. collected from Eastern Ghats, India. Science Research Reporter, 6(2):99-108.
Editor: Dr. Umesh P. Mogle, Printed, Published and Owned by Dr. Umesh P. Mogle, Published from ‘Kaushalyaaie’, P. No. 43, Priyanka Residancy, Mantha Chaufulli Jalna 431203 (M.S.) India.
Be a fan on Facebook
Google Scholar
RSS Feed
Follow us on LinkedIn
Doc Stoc
jsrr Blog