Green synthesis of silver nanoparticles using leaf extract of Lawsonia inermis and Psidium guajava and evaluation of their antibacterial activity

Dama L. B.,  Mane P. P., Pathan A. V., Chandarki  M. S., Sonawane S. R., Dama S. B., Chavan S. R. Chondekar R.P. and Vinchurkar A. S.

Biotechnology Research Laboratory, Department of Zoology, D.B.F. Dayanand College of Arts and Science, Solapur, (M.S.), India.
Department of Zoology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad. (M.S.), India.
E-mail: priyanka.mane33@gmail.com

Article Info		Abstract
Received: 23-06-2016 Revised: 21-08-2016 Accepted: 01-09-2016		The biosynthesis of silver nanoparticles has been proposed as a cost effective and environmental friendly alternative to chemical and physical methods. Plant mediated synthesis of nanoparticles is a green chemistry approach that interconnects nanotechnology and biotechnology. In the present study, synthesis of silver nanoparticles (AgNPs) has been performed using leaves extracts of two medicinal plants, Lawsonia inermis and Psidium guajava by reducing aqueous silver nitrate. Biologically synthesized silver nanoparticles have been widely used in the field of medicine. The silver nanoparticle formation was confirmed by the colour change of plant extracts. These silver nanoparticles were tested for antibacterial activity by using agar well diffusion method against the test organisms Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa and Bacillus subtilis. The antibacterial activity of silver nanoparticles was analysed by measuring the zone of inhibition. The results of present study revealed that the silver nanoparticles synthesized by Lawsonia inermis and Psidium guajava showed significant antibacterial activity against selected bacterial strains. Lawsonia inermis and Psidium guajava silver nanoparticles showed highest activity against Bacillus subtilis (13mm) and Escherichia coli (11mm) respectively. Therefore they have an important advantage over commercial antibiotics and may prevent the risk for generation of antibiotic resistant bacterial strains.
Keywords: Antibacterial activity, FTIR analysis, Medicinal plants, Silver nanoparticles.

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Dama L. B.,  Mane P. P., Pathan A. V., Chandarki  M. S., Sonawane S. R., Dama S. B., Chavan S. R. Chondekar R.P. and Vinchurkar A. S., 2016. Green synthesis of silver nanoparticles using leaf extract of Lawsonia inermis and Psidium guajava and evaluation of their antibacterial activity. Science Research Reporter, 6(2):89-95.

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